human duo set elisa kits (R&D Systems)
R&D Systems is a verified supplier 
R&D Systems manufactures this product 
94
Structured Review
R&D Systems
human duo set elisa kits
Human Duo Set Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/duoset+elisa+kit/Human+IL-1ra%2FIL-1F3+DuoSet+ELISA/pmc13122707-57-22-27
Average 94 stars, based on 45 article reviews
Human Duo Set Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/duoset+elisa+kit/Human+IL-1ra%2FIL-1F3+DuoSet+ELISA/pmc13122707-57-22-27
Average 94 stars, based on 45 article reviews
human duo set elisa kits - by Bioz Stars,
2026-09
94/100 stars
Images
1) Product Images from "C-terminal interleukin 1 alpha (IL-1α) overexpression drives EMT and a vulnerability to ferroptosis in HNSCC"
Article Title: C-terminal interleukin 1 alpha (IL-1α) overexpression drives EMT and a vulnerability to ferroptosis in HNSCC
Journal: Redox Biology
doi: 10.1016/j.redox.2026.104172
Figure Legend Snippet: Characterization of IL-1α overexpression constructs in Cal27 HNSCC cells. Gene expression (A-C), protein release (D) and protein expression (E-G) of the three IL1A constructs – Full- Length (FL), N-terminal (NT) and C-terminal (CT) in the Cal27 IL-1α-overexpressing cells were analyzed by RT-qPCR, ELISA and Western blot respectively compared to control. GAPDH was used as an endogenous control for PCR analysis (A-C); α-Tubulin was used as the loading control for whole cell lysate and cytoplasmic fraction western blots (E-F); and Lamin B1 was used as the loading control for the nuclear fraction Western blot (G). Bars represent mean ± SEM from n = 3 independent experiments. IL-1α band intensities were normalized to the respective loading controls. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.
Techniques Used: Over Expression, Construct, Gene Expression, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Western Blot, Control
Figure Legend Snippet: IL-1α overexpression alters expression of IL-1 pathway genes. Gene expression of IL1B (A), IL6 (B), IL8 (C), IL1RN (D), IL1RAP (E) and IL1R1 (F) in the three IL1A constructs – Full-Length (FL), N-terminal (NT) and C-terminal (CT) in Cal27 IL-1αoverexpressing cells were analyzed by RT-PCR using GAPDH as an endogenous control. Protein secretion of IL-1β (G), IL-6 (H), IL-8 (I), and IL1RA (J) in cell culture supernatants was quantified by ELISA with protein concentrations normalized to cell numbers. Whole cell lysates were analyzed for IL-1R1 expression by Western blot using GAPDH as a loading control (K). Bars represent mean ± SEM from n = 3 independent experiments.∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.
Techniques Used: Over Expression, Expressing, Gene Expression, Construct, Reverse Transcription Polymerase Chain Reaction, Control, Cell Culture, Enzyme-linked Immunosorbent Assay, Western Blot
Related Articles
Enzyme-linked Immunosorbent Assay:Article Title: Selective JAK Inhibition Reveals Paradoxical and Hierarchical Control of interferon-γ-driven Autoimmunity in AIRE Deficiency Article Snippet: ELISA buffer consisted of PBS supplemented with 0.05% Tween-20 and cOmpleteTM, Mini, EDTA-free Protease Inhibitor Cocktail (catalog no. 11836170001; Roche, USA). .. A Article Title: Recombinant antimicrobial multidomain polypeptide, methods of producing and uses thereof Article Snippet: An infection was simulated in HT29 cells with LPS (1 μg/mL) and the antimicrobial effect of HD5 present in JAMF1 protein compared to HD5 synthetic peptide (Peptanova) and recombinant HD5 fused to GFP reporter (HD5GFP) protein was assessed at 0.1 μM for 24 h. The antimicrobial effect was here monitorized through the stimulation of IL-8 in HT29 cells, being IL-8 a chemokine responsible to recruit neutrophils and phagocytes that kill pathogen bacteria. .. For IL-8 quantification, supernatants were collected from cells and levels of IL-8 were determined using Article Title: Spatiotemporal Atlas of Pro-Inflammatory (NF-κB) and Anti-Inflammatory (STAT6) Signalling Using Reporter Mice during mRNA Vaccination Article Snippet: Data acquisition was performed on a BD LSR Fortessa flow cytometer, and analysis was conducted using FlowJo software (Tree Star). .. The concentration of IL-12 in supernatants from BM-DCs was determined by ELISA using the Mouse IL-12/IL-23 p40 Allele-specific Article Title: Defined culture conditions improve functional properties of mature iPSC-derived macrophages for therapeutic screening Article Snippet: .. The concentration of secreted IL-6 was determined with the Article Title: Structure matters: commensal Phocaeicola vulgatus lipopolysaccharide induces attenuated microglial activation and preserves neuronal integrity Article Snippet: Cytokine levels (pg/mL) in BV2 and HMC3 supernatants were measured following E. coli or P. vulgatus LPS stimulation using the ultrasensitive ELLA-Simple Plex platform (Protein Simple, Bio-Techne, San José, CA, United States), with a commercially available microfluidic cartridge for mouse (ST01C-MP-004435) and for human (ST01A-PS-003229) IL-1β, IL-6, and TNF-α detection. .. IL-10 was quantified using Article Title: Defined culture conditions improve functional properties of mature iPSC-derived macrophages for therapeutic screening. Article Snippet: .. The concentration of secreted IL-6 was determined with the Article Title: Removal of volatile organic compounds by chemical filters significantly inhibited the development of atopic dermatitis symptoms in mice: Potential implications for air-conditioning systems in healthcare environments. Article Snippet: .. Briefly, LN single-cell suspensions (5 × 105 cells/well) were incubated with Dynabeads Mouse T-Activator CD3/CD28 (Thermo Fisher Scientific Inc., Kanagawa, Japan) for 24 or 96 h. Subsequently, the levels of IL-4, IL-13, and IL-17 in the supernatants were quantified using ELISA ( Article Title: Reduced plasma epidermal growth factor levels reflect poor glycaemic status in type 2 diabetes Article Snippet: The insulin levels were quantified using the Access Insulin Assay (Beckman Coulter, USA). .. EGF plasma levels were determined using the Concentration Assay:Article Title: Spatiotemporal Atlas of Pro-Inflammatory (NF-κB) and Anti-Inflammatory (STAT6) Signalling Using Reporter Mice during mRNA Vaccination Article Snippet: Data acquisition was performed on a BD LSR Fortessa flow cytometer, and analysis was conducted using FlowJo software (Tree Star). .. The concentration of IL-12 in supernatants from BM-DCs was determined by ELISA using the Mouse IL-12/IL-23 p40 Allele-specific Article Title: Defined culture conditions improve functional properties of mature iPSC-derived macrophages for therapeutic screening Article Snippet: .. The concentration of secreted IL-6 was determined with the Article Title: Defined culture conditions improve functional properties of mature iPSC-derived macrophages for therapeutic screening. Article Snippet: .. The concentration of secreted IL-6 was determined with the Single Cell:Article Title: Removal of volatile organic compounds by chemical filters significantly inhibited the development of atopic dermatitis symptoms in mice: Potential implications for air-conditioning systems in healthcare environments. Article Snippet: .. Briefly, LN single-cell suspensions (5 × 105 cells/well) were incubated with Dynabeads Mouse T-Activator CD3/CD28 (Thermo Fisher Scientific Inc., Kanagawa, Japan) for 24 or 96 h. Subsequently, the levels of IL-4, IL-13, and IL-17 in the supernatants were quantified using ELISA ( Incubation:Article Title: Removal of volatile organic compounds by chemical filters significantly inhibited the development of atopic dermatitis symptoms in mice: Potential implications for air-conditioning systems in healthcare environments. Article Snippet: .. Briefly, LN single-cell suspensions (5 × 105 cells/well) were incubated with Dynabeads Mouse T-Activator CD3/CD28 (Thermo Fisher Scientific Inc., Kanagawa, Japan) for 24 or 96 h. Subsequently, the levels of IL-4, IL-13, and IL-17 in the supernatants were quantified using ELISA ( Clinical Proteomics:Article Title: Reduced plasma epidermal growth factor levels reflect poor glycaemic status in type 2 diabetes Article Snippet: The insulin levels were quantified using the Access Insulin Assay (Beckman Coulter, USA). .. EGF plasma levels were determined using the |
![( a ) Cell viability was assessed with the MTT assay and data shown are means ± SD (n = 3). ( b ) <t>TNFα,</t> <t>IL-6</t> and IL-8 expression in response to B[a]P exposure. ( c ) Representative Western blot images showing protein expression. All data were obtained from three independent experiments. Statistical p values were determined by a one-way ANOVA, followed by a Tukey post-hoc test comparing the B[a]P treatments to the DMSO control; * p < 0.05; ¥, p < 0.01; NS, no significance.](https://bio-rxiv-images-cdn.bioz.com/dois_ending_with_31/10__64898_slash_2026__04__21__719931/10__64898_slash_2026__04__21__719931___F1.large.jpg)
![Cells were treated with various concentrations of PM2.5 for 24 hr. ( a ) Cell viability was assessed with MTT assay. ( b ) IL-6 and IL-8 from the basolateral sides. ( c ) Representative Western blot images showing CFTR, β2AR, phospho-AKT (Ser473), AKT, phospho-NF-κB p65 (Ser536), NF-κB p65, and TRPC6 expression. All data are presented as the mean ± SD (n = 4). Statistical p values were determined with a one-way ANOVA, followed by the Tukey post-hoc test comparing the B[a]P or PM 2.5 treatments to the DMSO control; *, p < 0.05; ¥, p < 0.01; NS, no significance.](https://bio-rxiv-images-cdn.bioz.com/dois_ending_with_31/10__64898_slash_2026__04__21__719931/10__64898_slash_2026__04__21__719931___F3.large.jpg)
![( a ) The lung parenchyma from two ferrets were sliced into several sections as shown. Arrows indicate the tracheobronchial tubes. All tissue sections were incubating with DMSO (control), 10 µM B[a]P, or 100 ng/mL PM 2.5 in Pneumacult-EX Plus Basal media for 24 hrs. ( b ) IL 6 secretion was determined using the ferret ELISA kit. Data represent the mean of 6 independent sections. Statistical p values were determined with a one-way ANOVA, followed by the Tukey post-hoc test comparing the B[a]P or PM 2.5 treatments to the DMSO control; ¥, p < 0.05 (n = 6). ( c ) Representative western blotting images show reduction of CFTR, and β2AR and elevation of TRPC6 expression in explants under these treatments.](https://bio-rxiv-images-cdn.bioz.com/dois_ending_with_31/10__64898_slash_2026__04__21__719931/10__64898_slash_2026__04__21__719931___F9.large.jpg)